Primer

Part:BBa_K4853007:Design

Designed by: Bhavya Guduru   Group: iGEM23_Virginia   (2023-10-12)


nisA forward primer and nisB forward primer


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

Sequence was optimized to increase the melting point and reach 50% GC content.


Source

This sequence was designed based on the nisA and nisB sequence via Benchling.

References